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In experiments carried out on cell cultures under special conditions, over 85 % of a breast cancer cell culture died off after 72 hours while no cytotoxicity was observed for a healthy cell culture.

The results not only indicate that histidine can increase methotrexate-driven cytotoxicity in cancer cells, but also raise the possibility that some mechanism is reducing the toxic effects of THF depletion in normal tissues.

D.M. established the expression cell lines and performed most cytotoxicity tests.

PD-L1 on tumor cells is sufficient for immune evasion in immunogenic tumors and inhibits CD8 T cell cytotoxicity.

However, its association with direct cytotoxicity is less strong.