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They plan to use McPherson’s strategy to assess more antibodies against other targets, gauging performance across three common assays: immunoblot, immunoprecipitation and immunofluorescence.

There are other methods researchers can try, too, such as coupling immunoprecipitation to mass spectrometry to see what proteins the antibody binds to4.

Back in 2012, our first task on the master’s programme was to carry out a chromatin immunoprecipitation assay to detect protein–DNA interactions in the cell nucleus.

Protein iBAQ values from the untagged control were subtracted from the tagged immunoprecipitation samples. h, Coomassie blue-stained gel of samples used for mass spectrometry analysis.

To measure bits of amyloid coursing through the bloodstream, Yanagisawa and colleagues used a technique called immunoprecipitation with mass spectrometry, which deploysantibodies to bind and identify proteins.