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Granddaddy called it the Proband.

For cases in which there is a single affected proband and no family history, investigators should consider sequencing the unaffected parents of the probands, permitting efficient discovery of de novo mutations and compound heterozygous genotypes.

Upon further medical evaluation, these mutations were found to account for each proband’s disease, leading to a change in diagnosis, some of which led to changes in patient management.

Australian pedigrees for which the proband was identified as carrying the E318K variant via Sequenom, as well as the panel of melanoma cell lines, were screened for E318K via Sanger sequencing using the following primers: forward, 5′-CAGGCTCGAGCTCATGGA-3′; reverse, 5′- TGGGGACACTATAGGCTTGG-3′.

If he fled from his lord's service, or from the land which he held, a writ issued de nativitate proband�, and the master recovered his fugitive by law.