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In module V, tropine and PLA glucoside are transported into the vacuole and together converted to littorine.

First, Srinivasan and Smolke engineered their strain to express a transporter protein from the tobacco plant Nicotiana tabacum that imports tropine into vacuoles.

The tropine produced in module II and the PLA glucoside from module III are imported into the vacuole.

In module II, putrescine is converted to tropine — the functional core that gives tropane alkaloids their name — through several cytosolic reactions, in addition to one catalysed in the peroxisome and another catalysed by an enzyme anchored to the membrane of the endoplasmic reticulum.

In addition to the types of compounds enumerated above we may also notice purin, tropine and the terpenes.